Related Experiment Video
Updated: Jul 19, 2025
11:27
A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
Published on: August 25, 2018
10.7K
NTRC regulates CP12 to activate Calvin-Benson cycle during cold acclimation
Jing Tsong Teh1, Verena Leitz2, Victoria J C Holzer1
1Department of Molecular Plant Science, Faculty of Biology, Ludwig-Maximilians-Universität Munich, Planegg 82152, Germany.
Summary
NADPH-dependent thioredoxin reductase C (NTRC) regulates chloroplasts and mediates cold tolerance. It reduces CP12, controlling a key enzyme complex essential for plant cold acclimation.
Area of Science:
- Plant Biology
- Biochemistry
- Molecular Biology
Background:
- Chloroplast redox regulation is vital for plant adaptation.
- NADPH-dependent thioredoxin reductase C (NTRC) is a key redox regulator in chloroplasts.
- Cold stress significantly impacts plant physiology and enzyme activity.
Purpose of the Study:
- To investigate the role of NTRC in cold tolerance in *Chlamydomonas reinhardtii*.
- To elucidate the molecular mechanism by which NTRC mediates cold acclimation.
- To identify interacting partners of NTRC involved in cold response.
Main Methods:
- Site-specific mutation analysis of NTRC active site.
- Coimmunoprecipitation and mass spectrometry to identify protein interactions.
- In vitro biochemical assays including size-exclusion chromatography.
- CRISPR-based gene editing to create mutant strains.
Main Results:
- NTRC mediates cold tolerance in a redox-dependent manner.
- NTRC directly reduces Calvin-Benson Cycle Protein 12 (CP12) in vitro.
- NTRC controls the integrity of the PRK/CP12/GAPDH complex, affecting enzyme activity in the cold.
- CP12 deletion impacts PRK and GAPDH protein levels, suggesting a protective role.
Conclusions:
- NTRC-mediated reduction of CP12 triggers the dissociation of the PRK/CP12/GAPDH complex in the cold.
- CP12 plays a dual role in enzyme complex integrity and protein protection, crucial for cold acclimation.
- This study reveals a critical redox-dependent mechanism for plant cold acclimation.
Related Concept Videos
01:46The Calvin Benson Cycle
4.6K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.6K
01:27C4 Pathway and CAM
45.7K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.7K
02:45Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
01:40The Calvin Cycle
74.9K
Overview
74.9K
01:57Adaptations that Reduce Water Loss
25.7K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.7K
01:28Global Regulatory Systems
44
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
44