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Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

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Depth-resolved method for attenuation coefficient calculation from optical coherence tomography data for improved

Alexander Moiseev¹, Evgeny Sherstnev¹, Elena Kiseleva²

¹Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod, Russia.

Journal of Biophotonics

|August 8, 2023

Summary

This summary is machine-generated.

Optical coherence tomography (OCT) helps determine tissue morphology. A new depth-resolved method accurately calculates attenuation coefficients, crucial for differentiating normal and pathological tissues, especially with sharp focusing.

Keywords:

attenuation coefficient brain tissue optical coherence tomography signal processing vulvar skin

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Area of Science:

Biomedical Optics
Medical Imaging
Ophthalmology

Background:

Optical coherence tomography (OCT) is valuable for intraoperative tissue morphology analysis.
Attenuation coefficient derived from OCT images can distinguish between normal and pathological tissues.
Existing methods face challenges with depth-dependent OCT sensitivity and imaging noise.

Purpose of the Study:

To adopt a depth-resolved method for calculating attenuation coefficients in real-world OCT scenarios.
To address challenges of depth-dependent sensitivity and additive imaging noise.
To evaluate the accuracy of attenuation coefficient calculation under different focusing conditions.

Main Methods:

Implemented a depth-resolved method for attenuation coefficient calculation.
Compensated for depth-dependent OCT sensitivity.
Analyzed the impact of scanning beam focus spot size (FWHM) on calculation accuracy.
Investigated additive imaging noise with a non-zero mean.

Main Results:

The proposed method accurately calculates attenuation coefficients without artifacts when using sharp focusing (≤10 μm FWHM).
With broader focusing (>10 μm FWHM), multiple calculation approaches are viable without significant bias.
Depth-dependent sensitivity compensation is crucial for accurate estimations with sharp focusing.

Conclusions:

Accurate depth-resolved attenuation coefficient estimation is achievable with appropriate methods.
The choice of OCT focusing impacts the required correction strategies for attenuation coefficient calculation.
Further research may refine methods for robust attenuation coefficient estimation in diverse OCT applications.