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DNA Markers, Pathogenicity Test, and Multilocus Sequence Analysis to Differentiate and Characterize Cereal-Specific
Eunhye Hong1, Ibukunoluwa A Bankole1, Bin Zhao2
1Department of Plant Pathology, North Dakota State University, Fargo, ND 58108, U.S.A.
Phytopathology
|August 8, 2023
Summary
New DNA markers efficiently differentiate Xanthomonas translucens pathovars undulosa (Xtu) and translucens (Xtt), crucial for managing bacterial leaf streak in wheat and barley. This aids disease control and resistance breeding efforts.
Area of Science:
- Plant Pathology
- Bacteriology
- Molecular Diagnostics
Background:
- Xanthomonas translucens comprises related bacterial pathogens classified into pathovars based on host specificity.
- Xanthomonas translucens pv. undulosa (Xtu) and Xanthomonas translucens pv. translucens (Xtt) cause significant bacterial leaf streak diseases in wheat and barley, respectively.
- Accurate differentiation of Xtu and Xtt is critical for disease management and epidemiological studies.
Purpose of the Study:
- To develop and validate DNA-based markers for the precise differentiation of Xtu and Xtt.
- To characterize a diverse collection of Xanthomonas translucens strains using these markers.
- To confirm pathovar identification through pathogenicity assays and multilocus sequence analysis/typing (MLSA/MLST).
Main Methods:
- Development of cleaved amplified polymorphic sequence (CAPS) markers based on single-nucleotide polymorphisms (SNPs).
- Design of Xtt-specific markers (Xtt-XopM and Xtt-SP1) derived from comparative genomics.
- Application of developed markers for strain identification, followed by pathogenicity tests and MLSA/MLST for validation.
Main Results:
- Successfully developed and utilized DNA markers to accurately identify Xtu and Xtt strains from a diverse collection.
- Pathogenicity tests and MLSA/MLST confirmed the pathovar assignments and revealed distinct genetic subclades within Xtu and Xtt.
- Identified seven sequence types for Xtu and three for Xtt, providing further genetic resolution.
Conclusions:
- Established efficient molecular methods for differentiating Xtu and Xtt, essential for accurate diagnosis.
- Characterized Xtu and Xtt strains, providing valuable data for understanding disease epidemiology and host-pathogen interactions.
- The developed markers and strain characterization support breeding programs seeking resistance to these important bacterial pathogens.
