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Bilirubin diffusion through lipid membranes.

D Hayward, D Schiff, S Fedunec

    Biochimica Et Biophysica Acta
    |August 7, 1986
    PubMed
    Summary
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    Bilirubin can pass through lipid membranes, such as liposomes. Its diffusion rate changes with lipid phase transitions and albumin concentrations, indicating free membrane permeability.

    Area of Science:

    • Biochemistry
    • Biophysics
    • Cell Biology

    Background:

    • Bilirubin, a breakdown product of heme, can accumulate in tissues.
    • Understanding bilirubin transport across cell membranes is crucial for treating conditions like neonatal jaundice.

    Purpose of the Study:

    • To investigate the diffusion of bilirubin across lipid bilayers.
    • To determine if bilirubin can permeate liposomes composed of different lipid compositions.

    Main Methods:

    • Liposomes made from dipalmitoylphosphatidylcholine (DPPC), egg phosphatidylcholine (egg PC) with cholesterol, and neuroblastoma cell lipids were used.
    • Internalized bilirubin and albumin were studied for efflux into external albumin solutions.
    • Bilirubin efflux was measured across lipid membranes under varying conditions.

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    Main Results:

    • Bilirubin efflux from DPPC liposomes increased significantly above the lipid phase transition temperature.
    • The phase transition, not just temperature, influenced bilirubin diffusion.
    • Bilirubin efflux from egg PC-cholesterol liposomes indicated an equilibrium distribution dependent on albumin concentrations.

    Conclusions:

    • Bilirubin demonstrates free diffusion across the investigated lipid membranes.
    • Lipid phase transitions and albumin presence modulate bilirubin membrane permeability.
    • These findings have implications for bilirubin transport mechanisms in biological systems.