Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

5.8K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
5.8K
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones01:15

NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones

4.2K
In aldehydes, the hydrogen atom connected to the carbonyl carbon helps distinguish aldehydes from other carbonyl compounds using ¹H NMR spectroscopy. The closeness of aldehydic hydrogen to the electrophilic carbonyl carbon highly deshields the hydrogen atom causing its signal to appear around 10 ppm in the ¹H NMR spectra. α hydrogens split the aldehydic proton signal, which helps identify the number of α hydrogens in the molecule. For instance, one α hydrogen creates a...
4.2K
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

617
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
617
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

416
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
416
Oxidations of Aldehydes and Ketones to Carboxylic Acids01:15

Oxidations of Aldehydes and Ketones to Carboxylic Acids

4.0K
Oxidation of aldehydes and ketones results in the formation of carboxylic acids. Aldehydes, bearing hydrogen next to the carbonyl group, are easily oxidized compared to ketones. This is because an aldehydic proton can easily be abstracted during oxidation.
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...
4.0K
Mass Spectrometry: Aldehyde and Ketone Fragmentation01:09

Mass Spectrometry: Aldehyde and Ketone Fragmentation

3.4K
In mass spectrometry, the fragmentation of aliphatic aldehydes and ketones generally occurs through three key mechanisms: α-cleavage, inductive cleavage, and the McLafferty rearrangement.
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fortified Eggs as Food-Based Vehicles for Nutrient Delivery: A Scoping Review of Human Intervention Studies.

Nutrients·2026
Same author

Plasmonic trimer nanoarrays with probe-trapping sites for SERS detection of urinary copper in Wilson's disease.

The Analyst·2026
Same author

High-throughput DNA engineering by mating bacteria.

Cell systems·2026
Same author

Design, Synthesis, Antifungal Activity, and Action Mechanism of Novel l-Perillyl Alcohol Derivatives Containing a Piperazine Moiety.

Journal of agricultural and food chemistry·2026
Same author

HIF-2α/TRIB3/PARP1 axis promotes progression by orchestrating DNA repair in clear cell renal cell carcinoma.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

Research waste among randomized controlled trials of delirium: A cross-sectional study.

International psychogeriatrics·2026

Related Experiment Video

Updated: Jul 19, 2025

Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out
07:10

Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out

Published on: November 9, 2018

9.5K

Pattern Recognition and Visual Detection of Aldehydes Using a Single ESIPT Dye.

Liangfei Fan1, Weiyi Li1, Dongmin Jia1

  • 1Department of Chemistry, College of Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

Analytical Chemistry
|August 9, 2023
PubMed
Summary

Researchers developed a novel fluorescent sensor for aldehyde detection. The sensor, BS1, effectively distinguishes and quantifies aldehydes, even in vapor form, by utilizing pH-mediated fluorescence changes.

More Related Videos

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
09:59

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

Published on: March 31, 2023

1.1K
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.6K

Related Experiment Videos

Last Updated: Jul 19, 2025

Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out
07:10

Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out

Published on: November 9, 2018

9.5K
Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
09:59

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

Published on: March 31, 2023

1.1K
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.6K

Area of Science:

  • Analytical Chemistry
  • Organic Chemistry
  • Materials Science

Background:

  • Accurate discrimination and quantification of aldehydes are challenging due to their similar chemical reactivities.
  • Manipulating reaction pH can alter aldehyde reactivity and Schiff base stability for analytical applications.

Purpose of the Study:

  • To design and synthesize novel benzothiazole-based fluorescent molecules for aldehyde sensing.
  • To investigate the influence of substituent positions on aldehyde detection capabilities.
  • To develop a pH-mediated sensor array for reliable aldehyde discrimination and identification.

Main Methods:

  • Synthesis of three benzothiazole-based fluorescent molecules (BS1-BS3) with varying amino group positions.
  • Evaluation of fluorescence response in the presence of formaldehyde at different pH levels.
  • Construction of a pH-mediated sensor array (BS1SA) using the most responsive molecule (BS1).

Main Results:

  • Only BS1, with an ortho-substituted amino group, showed a significant fluorescence response to formaldehyde, attributed to Excited-State Intramolecular Proton Transfer (ESIPT).
  • BS2 and BS3 exhibited negligible responses, highlighting the importance of substituent position.
  • The BS1SA demonstrated reliable discrimination of seven different aldehydes and identification of unknown samples.
  • BS1 was successfully used to create a fluorescent test paper for visual detection of formaldehyde vapor.

Conclusions:

  • The ortho-amino substituted benzothiazole derivative (BS1) is a highly effective fluorescent probe for aldehyde sensing.
  • A pH-mediated sensor array (BS1SA) offers a robust platform for aldehyde discrimination and identification.
  • The developed fluorescent test paper provides a simple and visual method for formaldehyde vapor detection.