Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CSF1R regulates monocyte subset differentiation and intracellular metabolism.

Nature communications·2026
Same author

Novel mouse models for perianal fistulizing Crohn's disease reveal therapeutic value of interferon-γ antagonists.

bioRxiv : the preprint server for biology·2026
Same author

Oxaliplatin triggers adipose and muscle wasting through cancer-independent metabolic and CNS pathways in mouse models.

Communications biology·2026
Same author

Oral [<sup>18</sup>F]-Fluoro-Thia-Heptadecanoic Acid Positron Emission Tomography Reveals Mesenteric-to-Central Lymphatic Flow.

Gastro hep advances·2026
Same author

Inflammaging in aged tissues drives remodeling of the CD8<sup>+</sup> T cell compartment.

Cell reports·2026
Same author

mRNA vaccines engage unconventional pathways in CD8<sup>+</sup> T cell priming.

Nature·2026

Related Experiment Video

Updated: Jul 19, 2025

Isolation of Murine Lymph Node Stromal Cells
05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

31.4K

Resident dendritic cell density in the lymph node paracortex is preDC-estined.

Rafael S Czepielewski1, Gwendalyn J Randolph2

  • 1Immunology Center of Georgia, Medical College of Georgia, Department of Physiology, Augusta University, Augusta, GA, USA.

Immunity
|August 9, 2023
PubMed
Summary

Type 1 conventional dendritic cells (cDC1s) are maintained in lymph nodes through proliferation of precursor dendritic cells (preDCs) in medullary vessels. Flt3L signaling regulates the transition from preDC to mature cDC1, ensuring stable cell numbers.

More Related Videos

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
09:07

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Published on: September 19, 2013

19.3K
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
08:12

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Published on: March 20, 2013

11.9K

Related Experiment Videos

Last Updated: Jul 19, 2025

Isolation of Murine Lymph Node Stromal Cells
05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

31.4K
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
09:07

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Published on: September 19, 2013

19.3K
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
08:12

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Published on: March 20, 2013

11.9K

Area of Science:

  • Immunology
  • Cell Biology
  • Lymphatic System

Background:

  • Dendritic cells (DCs) are crucial immune cells with short lifespans.
  • Despite their short lifespan, dendritic cell populations in lymph nodes remain stable.
  • The mechanisms maintaining stable dendritic cell numbers require further elucidation.

Purpose of the Study:

  • To investigate the origin and maintenance mechanisms of type 1 conventional dendritic cells (cDC1s) in lymph nodes.
  • To identify the cellular precursors and anatomical locations involved in cDC1 homeostasis.
  • To understand the regulatory signals governing the differentiation of dendritic cell precursors.

Main Methods:

  • Utilized advanced imaging techniques to track dendritic cell populations in vivo.
  • Employed genetic lineage tracing to identify precursor cell populations.
  • Analyzed the role of Flt3L signaling in dendritic cell development and maintenance.

Main Results:

  • Identified a precursor dendritic cell (preDC) population residing in medullary vessels.
  • Demonstrated that these preDCs proliferate and migrate to the deep paracortex.
  • Showed that Flt3L sensing is critical for the transition of preDCs to cDC1s.

Conclusions:

  • Lymph node cDC1 populations are sustained by a proximally located, proliferating precursor pool.
  • The differentiation of precursor dendritic cells into cDC1s is tightly regulated by Flt3L.
  • This study reveals a novel mechanism for maintaining immune cell homeostasis within lymphoid organs.