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Updated: Jul 19, 2025
Isolation of Primary Murine Retinal Ganglion Cells RGCs by Flow Cytometry
Published on: July 5, 2017
Purification of Retinal Ganglion Cells from Differentiation Through Adult via Immunopanning and Low-Pressure Flow
Sean M Riordan1, Afnan M Aladdad1, Kiran J McLoughlin1,2
1Department of Ophthalmology and Department of Biomedical Sciences, University of Missouri-Kansas City, Kansas City, MO, USA.
Abstract:
The isolation and culturing of rodent retinal ganglion cells (RGC) is a key step in studying the function and cellular response of this crucial cell type. Typical methods used for isolation of RGCs include immunopanning or magnetic bead separation with antibodies targeting RGC specific protein markers. However, in developmental research, many of the most common markers, such as Thy-1, are not expressed in early stages of development. To help study these crucial early stage RGCs, we have developed a novel method that utilizes a transgenic mouse with a GFP tag on the protein BRN3 and a low-pressure fluorescence-activated cell sorter (FACS) system.
