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Sperm Structure and Semen Composition

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During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...

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Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

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Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams.

Alejandro Jurado-Campos¹, Pedro Javier Soria-Meneses¹, María Arenas-Moreira^2,3

¹SaBio IREC (CSIC-UCLM-JCCM), Campus Universitario, S/N, 02071, Albacete, Spain.

Journal of Animal Science and Biotechnology

|August 9, 2023

Summary

This summary is machine-generated.

Vitamin E nanoemulsion effectively preserves ram sperm quality during transport for up to 96 hours at 15-22°C. This nanotechnology enhances sperm viability and motility, offering greater flexibility for artificial insemination programs.

Keywords:

Breeding technology Nanoemulsions Nanotechnology Sperm oxidative stress Vitamin E

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Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Area of Science:

Reproductive Biology
Nanotechnology in Animal Science
Sperm Preservation

Background:

Artificial insemination (AI) in animals is limited by sperm sensitivity to temperature and short fertile lifespan.
Nanotechnology offers novel solutions for sperm protection during AI.
Vitamin E nanoemulsion (NE) was investigated for improving ram sperm quality during transport.

Purpose of the Study:

To evaluate the efficacy of vitamin E nanoemulsion (NE) in preserving ram sperm quality.
To assess the impact of NE on sperm motility, viability, and integrity during storage.
To determine the feasibility of using NE for extended sperm transport in AI.

Main Methods:

Ram ejaculates were collected and extended with Andromed®.
Samples were treated with vitamin E NE (12 mmol/L) or control, with/without oxidative stress.
Sperm parameters (motility, viability, acrosomal integrity, mitochondrial potential, lipoperoxidation, ROS, DNA integrity) were monitored over 96 hours at 15°C and 22°C.

Main Results:

Vitamin E NE maintained ram sperm quality for up to 96 hours at both 15°C and 22°C.
No significant loss in kinematic or physiological characteristics was observed in NE-treated samples.
NE demonstrated protective effects against oxidative stress, preserving sperm parameters.

Conclusions:

Vitamin E nanoemulsions can be used for liquid storage of ram spermatozoa for 2-5 days.
This method offers increased flexibility for breeders in artificial insemination programs.
Further research is needed to assess the fertility outcomes of AI using NE-preserved sperm.