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Related Concept Videos

Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
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Cell Adhesion in Plants01:14

Cell Adhesion in Plants

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Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
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Cancer Cell Migration through Invadopodia01:35

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Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
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Selectins01:25

Selectins

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Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
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Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

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In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
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Related Experiment Video

Updated: Jul 19, 2025

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
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Reducing Cancer Cell Adhesion using Microtextured Surfaces.

Caroline McCue1, Adel Atari2, Sean Parks1

  • 1Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA, 02139, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|August 9, 2023
PubMed
Summary

Microtextured surfaces reduce cell adhesion, offering a new approach for cell culture. This method improves cell detachment efficiency while maintaining cell viability and proliferation for advanced cell culture applications.

Keywords:
cell adhesioncell morphologymicrofluidicsmicrotexturepolystyrene

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Area of Science:

  • Biomaterials Engineering
  • Cell Biology
  • Surface Science

Background:

  • Trypsinization has been the standard for cell dissociation for over a century.
  • Current enzymatic methods for large-scale cell culture are labor-intensive and generate waste.
  • There is a need for improved cell culture surfaces supporting delicate cells and high-throughput workflows.

Purpose of the Study:

  • To engineer microtextured polystyrene (PS) surfaces to reduce cell adhesion strength.
  • To evaluate the impact of microposts on cell adhesion, viability, and proliferation.
  • To assess the efficiency of cell detachment from microtextured surfaces.

Main Methods:

  • Fabrication of microtextured polystyrene surfaces with microposts.
  • Cell viability and proliferation assays using two cancer cell lines.
  • Cell image analysis to quantify changes in cell morphology.
  • Microfluidic-based cell detachment experiments comparing micropost and flat surfaces.

Main Results:

  • Microtextured surfaces showed comparable cell viability and proliferation to standard cell culture vessels.
  • Cells on micropost surfaces exhibited reduced area and increased length per area.
  • Microfluidic methods demonstrated up to a sevenfold greater percentage of cell removal from micropost surfaces compared to flat controls.

Conclusions:

  • Engineered micropost surfaces effectively decrease cell adhesion strength.
  • Microtextured surfaces maintain cell viability and proliferation, similar to conventional methods.
  • These surfaces offer a promising alternative for advanced cell culture and automated workflows.