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Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

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Published on: January 17, 2012

Progranulin modulates the progression of non-small cell lung cancer through lncRNA H19

Biao Gu^1,2, Maoyuan Yang¹, Liang Shi¹

¹Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University 899 Pinghai Road, Suzhou 215006, Jiangsu, China.

American Journal of Translational Research

|August 10, 2023

Abstract

Objective:

This study aimed to explore the specific mechanism of action of Progranulin (PGRN) in non-small cell lung cancer (NSCLC) and its interaction with lncRNA H19.

Methods:

Normal and cancerous lung tissues were collected from patients with NSCLC and healthy volunteers. We assessed the expression of PGRN in both groups using immunohistochemistry, quantitative-reverse transcription-polymerase chain reaction (qRT-PCR), and western blotting (WB).

Results:

Compared to the controls, PGRN expression was noticeably higher in tumor tissues. The high expression of PGRN in patients with NSCLC was inversely correlated to the prognosis and strongly associated with the biological features and clinicopathologic data. High PGRN expression significantly improved the ability of NSCLC cells to proliferate and migrate and was positively correlated with tumor formation, based on in vitro and in vivo cellular tests. Expression of lncRNA H19 was also found to be elevated in NSCLC tissue and cells. The expression of H19 was correlated with tumor growth in vivo and in vitro, and H19 regulated PGRN by mediating the expression of miR-29b-3p.

Conclusions:

H19 and PGRN can serve as biomarkers and therapeutic targets in NSCLC.

Keywords:

H19 Non-small cell lung cancer miR-29b-3p progranulin (PGRN)proliferation

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