Study on the protective effect of chondroitin sulfate from sturgeons on rat chondrocytes and its potential mechanisms

To investigate the protective effect of Chondroitin Sulfate from Sturgeons on rat chondrocytes and its possible mechanism.

The model of chondrocyte injury induced by hydrogen peroxide was established and chondrocytes were cultured and divided into the following groups: control group, sham group, model group, Sofast group, Low dose of Chondroitin Sulfate from Sturgeon B (CSSB-L) group, Moderate dose of Chondroitin Sulfate from Sturgeon B (CSSB-M) group and High dose of Chondroitin Sulfate from Sturgeon B (CSSB-H) group. The cell proliferation was analyzed by Cell Counting Kit-8 (CCK-8) assay. The cell apoptosis was detected by flow cytometer. The expression levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8) and Interferon gamma (IFN-γ) in cell supernatants were examined by Enzyme-linked immunosorbent assay (ELISA). Western blot analysis was used to detect the levels of proteins associated with Wnt signal pathway in chondrocytes.

Compared with the control group and sham group, the cell proliferation was decreased significantly, cell apoptosis was increased obviously, and the levels of IL-6, IL-8 and IFN-γ were remarkably increased in the model group. For Wnt signal pathway related proteins, the levels of Wnt3a, Frizzled5, Dsh, β-Catenin and C-myc proteins in the model group were significantly reduced, and p-GSK3β expression level was obviously increased (all P<0.05). Compared with the model group, CSSB could promote cell viability, and inhibit cell apoptosis and the levels of IL-6, IL-8 and IFN-γ (all P<0.05). The levels of Wnt signaling pathways related proteins in the CSSB-M group and CSSB-H group were obviously expressed.

Chondroitin sulfate from sturgeons protected rat chondrocytes from injuries induced by hydrogen peroxide, which may be associated with the Wnt signaling pathway.