Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.4K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural dynamics of RNA polymerase II throughout the nucleotide addition cycle.

Nature communications·2026
Same author

Structural Dynamics of RNA Polymerase II During Nucleotide Addition Cycle.

bioRxiv : the preprint server for biology·2026
Same author

Structural Advances in Respiratory Syncytial Virus: Implications for Vaccine and Antiviral Development.

Microorganisms·2026
Same author

Integrase anchors viral RNA to the HIV-1 capsid interior.

Nature·2026
Same author

<i>In situ</i> chromatin dynamics and HIV-1 nuclear trafficking.

bioRxiv : the preprint server for biology·2025
Same author

Uncovering structural determinants of peptide recognition by public and private T-cell receptors.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jul 19, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.1K

Cryo-electron tomography to study viral infection.

Miles Graham1, Peijun Zhang1,2,3

  • 1Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, U.K.

Biochemical Society Transactions
|August 10, 2023
PubMed
Summary

Cryo-electron tomography (cryo-ET) advances structural virology by visualizing viruses and host interactions. This review details cryo-ET workflows for studying viral infection biology and future potential.

Keywords:
in situcorrelative microscopycryo-EMcryo-electron tomographysubtomogram averagingvirus infection

More Related Videos

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

12.3K
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
08:31

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Published on: July 20, 2022

3.1K

Related Experiment Videos

Last Updated: Jul 19, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.1K
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo
08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

12.3K
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
08:31

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Published on: July 20, 2022

3.1K

Area of Science:

  • Structural biology
  • Virology
  • Microscopy

Background:

  • Cryo-electron microscopy (cryo-EM) has historically aided virus studies.
  • Single particle cryo-EM achieved success, paving the way for cryo-electron tomography (cryo-ET).
  • Cryo-ET is rapidly advancing structural virology.

Conclusions:

  • Cryo-ET is a powerful tool for advancing structural virology.
  • The technology enables detailed characterization of viral infection processes.
  • Future developments will further unlock cryo-ET's potential in understanding viral mechanisms.