Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

1.3K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
1.3K
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

282
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
282

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Second-Harmonic Generation Imaging of Humidity-Induced Salt Crystallization in Spider Aggregate Glue.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Stable deep-blue photoluminescence from photoirradiated cyanobiphenyl liquid crystals.

Chemical communications (Cambridge, England)·2026
Same author

Usefulness of an S-1 dosage formula for predicting adverse events in adjuvant chemotherapy for curatively resected pancreatic cancer: an exploratory analysis of the randomized clinical trial (JASPAC-01).

Journal of gastroenterology·2026
Same author

Rapid identification of microplastics in complex biological matrices via high-speed mid-infrared hyperspectral imaging.

Talanta·2026
Same author

Generation of spectrally narrow, phase-stable pulse trains using a birefringent crystal array.

Optics express·2026
Same author

Sub-10-nJ femtosecond mode-locked oscillator based on a double-clad thulium-doped fluoride fiber.

Optics express·2026

Related Experiment Video

Updated: Jul 19, 2025

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.0K

Mid-Infrared Optical Force Chromatography of Microspheres Containing Siloxane Bonds.

Yoshua Albert Darmawan1, Takuma Goto1, Taiki Yanagishima2

  • 1Laser Science Laboratory, Toyota Technological Institute, 2-12-1 Hisakata, Tempaku-ku, Nagoya 468-8511, Japan.

The Journal of Physical Chemistry Letters
|August 10, 2023
PubMed
Summary

This study introduces mid-infrared optical forces for sorting microparticles by molecular vibrations. The technique precisely separates microspheres based on their absorbance, enabling optical property estimation.

More Related Videos

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.1K
Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
11:34

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

Published on: December 20, 2013

7.3K

Related Experiment Videos

Last Updated: Jul 19, 2025

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

13.0K
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

11.1K
Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
11:34

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

Published on: December 20, 2013

7.3K

Area of Science:

  • Optics
  • Materials Science
  • Spectroscopy

Background:

  • Optical forces offer label-free separation of micro- and nanomaterials.
  • Mid-infrared (MIR) spectroscopy probes molecular vibrations, crucial for material identification.
  • Precise manipulation of microparticles is essential for various scientific and technological applications.

Purpose of the Study:

  • To develop and demonstrate a mid-infrared optical force technique for sorting microspheres.
  • To correlate microsphere sorting with their molecular vibrational properties and absorbance.
  • To enable estimation of optical properties of individual microspheres using their manipulated motion.

Main Methods:

  • Utilizing a mid-infrared quantum cascade laser to generate an evanescent field at 9.3 μm.
  • Employing total internal reflection on a prism for optical force generation.
  • Analyzing microsphere displacement and velocity under optical manipulation.
  • Finite-difference time-domain (FDTD) simulations for optical force calculations.

Main Results:

  • Successful sorting of various microspheres, including those with Si-O-Si bonds, based on their 9.3 μm absorbance.
  • Experimental results align well with FDTD optical force calculations.
  • Demonstrated prediction of microsphere displacement and velocity from absorbance values.
  • Showcased the ability to estimate microsphere optical properties (absorbance, complex refractive index) from velocity.

Conclusions:

  • Mid-infrared optical forces provide a precise method for sorting microparticles based on molecular vibrations.
  • This technique allows for non-invasive characterization of individual microsphere optical properties.
  • The developed method has potential applications in materials analysis, diagnostics, and microparticle manipulation.