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Deficiency of circ_0103809 Attenuates Non-small Cell Lung Cancer Malignant Progression by Controlling

Xueliang Yang1, Xinchun Chai1, Yongming Song1

  • 1Department of Thoracic Surgery, Shanxi Hospital Affiliated to Cancer Hospital, Shanxi Province Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical University, No. 3, Beihewan East Street, Worker Street, Xinghualing District, Taiyuan, Shanxi, China.

Biochemical Genetics
|August 10, 2023
PubMed

Insights

Circular RNA circ_0103809 promotes non-small cell lung cancer (NSCLC) progression by sponging miR-153-3p and upregulating HDAC1. Circ_0103809 may serve as a diagnostic biomarker for NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer development.
  • Non-small cell lung cancer (NSCLC) is a major cause of cancer-related mortality, necessitating novel diagnostic and therapeutic targets.

Purpose of the Study:

  • To investigate the role and underlying mechanism of circ_0103809 in the progression of non-small cell lung cancer (NSCLC).
  • To evaluate the potential of circ_0103809 as a diagnostic biomarker for NSCLC.

Main Methods:

  • Quantitative real-time PCR and western blot to assess expression levels of circ_0103809, miR-153-3p, and HDAC1.
  • In vitro assays (MTT, EdU, flow cytometry, wound healing, tube formation) to analyze functional roles.
  • Dual-luciferase, RIP, and pull-down assays to confirm molecular interactions.
  • In vivo animal models to validate findings.

Main Results:

  • Circ_0103809 was significantly upregulated in NSCLC tissues, cells, and serum-derived exosomes, showing potential as a diagnostic biomarker.
  • Silencing circ_0103809 inhibited NSCLC cell proliferation, migration, angiogenesis, and induced apoptosis and cell cycle arrest.
  • Circ_0103809 functioned as a miR-153-3p sponge, and miR-153-3p targeted HDAC1, thereby inhibiting NSCLC malignancy.
  • Knockdown of circ_0103809 suppressed tumor growth in vivo.

Conclusions:

  • Circ_0103809 promotes NSCLC progression by modulating the miR-153-3p/HDAC1 axis.
  • Circ_0103809 represents a potential diagnostic biomarker and therapeutic target for NSCLC.
Keywords:
ExosomeHDAC1NSCLCcirc_0103809miR-153-3p

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