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[Determination of DNA level in human plasma]
Biulleten' Eksperimental'Noi Biologii I Meditsiny
|September 1, 1986
Summary
Healthy donors have 5-30 μg/ml plasma DNA, comparable to leukocyte DNA content. A novel fluorescence method accurately quantifies this circulating DNA in small plasma volumes.
Area of Science:
- Biochemistry
- Molecular Biology
- Clinical Chemistry
Context:
- Circulating cell-free DNA (cfDNA) in plasma is a significant biomarker.
- Accurate quantification of plasma DNA is crucial for diagnostic applications.
- Existing methods for DNA quantification can be complex or require larger sample volumes.
Purpose:
- To establish the normal range of DNA concentration in plasma of healthy individuals.
- To develop and validate a simple, specific method for plasma DNA quantification.
- To determine the feasibility of using small plasma volumes for DNA analysis.
Summary:
- Demonstrated that plasma DNA concentration in healthy donors ranges from 5 to 30 μg/ml.
- This concentration is equivalent to 10-50% of the DNA content found in leukocytes from the same blood volume.
- Developed a method using DNA-bisbenzimide complex fluorescence in a 10% NaCl plasma supernatant, heated to 100°C for 2-3 minutes, to measure DNA concentration.
- The method is sensitive, specific, and requires only 0.05-0.1 ml of plasma.
Impact:
- Provides a baseline reference for plasma DNA levels in healthy populations.
- Offers a simplified and accessible method for quantifying circulating DNA.
- Enables cfDNA analysis from minimal plasma samples, potentially improving accessibility for various clinical and research settings.