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Updated: Jul 19, 2025

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
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Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

Yan Huang1,2,3, Shuping Chen1,2,3, Yao Yao1,2,3

  • 1Jiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, China.

Journal of Agricultural and Food Chemistry
|August 11, 2023
PubMed

Insights

Ovotransferrin (OVT) effectively reduces gastric inflammation by inhibiting key inflammatory cytokines like IL-8, IL-6, and TNF-α. This natural protein works by suppressing the MAPK and NF-κB signaling pathways, showing potential for treating gastric inflammation.

Area of Science:

  • Gastroenterology and Immunology
  • Biochemistry and Molecular Biology

Background:

  • Ovotransferrin (OVT) possesses known anti-inflammatory properties.
  • The specific mechanisms of OVT's action on gastric inflammation remain largely uncharacterized.

Purpose of the Study:

  • To investigate the anti-inflammatory effects of Ovotransferrin (OVT) on gastric epithelial cells (GES-1) stimulated with tumor necrosis factor-α (TNF-α).
  • To elucidate the underlying molecular mechanisms, particularly the involvement of MAPK and NF-κB signaling pathways.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) for quantifying inflammatory cytokines.
  • RNA sequencing to identify affected cellular pathways.
  • Western blotting and pathway inhibitors to validate molecular mechanisms.

Main Results:

  • Ovotransferrin (OVT) demonstrated no toxicity to GES-1 cells at tested concentrations (50-400 μg/mL).
  • OVT significantly reduced the secretion of interleukin-8 (IL-8), IL-6, and TNF-α.
  • RNA sequencing and subsequent validation confirmed OVT's inhibition of mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain enhancer of activated B cell (NF-κB) pathways.

Conclusions:

  • Ovotransferrin (OVT) exhibits significant anti-inflammatory effects in gastric epithelial cells.
  • OVT exerts its protective effects by modulating the MAPK and NF-κB signaling cascades.
  • OVT is a promising natural protein therapeutic candidate for managing gastric inflammation.
Keywords:
GES-1 cellsMAPK and NF-κB pathwaysRNA-seq analysisanti-inflammationovotransferrin

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