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Updated: Jul 19, 2025
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Published on: April 16, 2013
Single laboratory evaluation of umbilical cord blood units processing methodologies for banking
Francisco F Dos Santos1, Letícia Nunes1, Cátia Martins1
1Stemlab, FamiCord Group, Cantanhede, Portugal.
Objective:
To compare the efficiency of 3 different processing methods (Sepax, AutoXpress [AXP], and manual processing with hydroxyethyl starch [HES] sedimentation) used at Stemlab during a 10-year period.
Methods:
Historical data were compiled and the analytical results obtained for the 3 different methods were compared.
Results:
The manual processing (HES) method yielded the highest level of total nucleated cell recovery after processing, and the AXP system yielded the highest CD34+ cell number. The red blood cell reduction was also significantly higher with the HES method. Also, HES showed comparable results to Toticyte technology for umbilical cord blood (UCB) processing.
Conclusion:
These results show that the HES method is as effective as automated technologies for UCB volume reduction; hence, it is a suitable methodology for private and public UCB banks. The HES method also proved to be superior to Toticyte technology for medical applications, with higher recovery yields of total nucleated cells after thawing and equivalent CD34+ cell recovery and functionality.
