Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impaired glycoRNA biogenesis in metabolic-dysfunction associated steatotic liver disease.

Journal of hepatology·2026
Same author

Tumor sialylation regulates G-CSF stability and promotes neutrophil-mediated immunosuppression in breast cancer.

Nature communications·2026
Same author

JAM-A as a potential surface marker of human pluripotent stem cells.

Journal of proteomics·2026
Same author

In planta engineering of polysialylated glycoproteins using salmonid polysialyltransferases.

Scientific reports·2026
Same author

The Enzymatic Mechanism of OAS: How Metal Ions and Quantum Effects Help Activate Innate Immunity.

ACS omega·2026
Same author

In silico typing maps the natural diversity of Escherichia coli transporter-dependent capsules.

Nature microbiology·2026
Same journal

The rheological behavior, particle properties and supramolecular structure of low acyl gellan gum fluid gels: impact of the calcium concentration before fluid gel formation.

Carbohydrate polymers·2026
Same journal

Tuning the degree of substitution of octenyl succinic anhydride-modified highly branched cyclodextrin: Impacts on structure, physicochemical properties and emulsifying performance.

Carbohydrate polymers·2026
Same journal

Tailoring cellulose nanofibril separator networks with lignin for sustainable energy storage.

Carbohydrate polymers·2026
Same journal

Dual-functional synergistic modification of cellulose with phosphate and amidoxime for high-efficiency uranium capture in acidic wastewater.

Carbohydrate polymers·2026
Same journal

Hierarchical construction of a superhydrophobic and superior intrinsically flame-retardant phosphorylated microcrystalline cellulose bio-based composite aerogel.

Carbohydrate polymers·2026
Same journal

Structural characterization, in vitro anticoagulant, and antiplatelet activities of a Distolasterias nipon dermatan sulfate-like polymer with a distinctive sulfation pattern.

Carbohydrate polymers·2026
See all related articles

Related Experiment Video

Updated: Jul 19, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.8K

Extending the enzymatic toolbox for heparosan polymerization, depolymerization, and detection.

Małgorzata Sulewska1, Monika Berger2, Manuela Damerow2

  • 1Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany; Centre de Recherche sur les Macromolécules Végétales, Groupe Chimie et Biotechnologie des Oligosaccharides, 601 rue de la Chimie, BP 53X, 38041 Grenoble, Cedex 09, France.

Carbohydrate Polymers
|August 11, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed new methods to detect heparosan, an important polysaccharide, using enzymes from E. coli K5 and bacteriophage ΦK5B. This breakthrough enables better detection of heparosan in various biological samples for pharmaceutical applications.

Keywords:
BacteriophageEnzymatic synthesisGlycosaminoglycansHeparosanLyaseTailspike protein

More Related Videos

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

14.2K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

Related Experiment Videos

Last Updated: Jul 19, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.8K
DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

14.2K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K

Area of Science:

  • Biochemistry
  • Microbiology
  • Polymer Science

Background:

  • Heparosan is an acidic polysaccharide crucial for heparan sulfate and heparin biosynthesis, showing high biocompatibility for pharmaceutical uses.
  • Detecting heparosan in biological samples is challenging due to its low immunogenicity, hindering antibody development.

Purpose of the Study:

  • To develop novel methods for controlled synthesis and depolymerization of heparosan.
  • To enable the detection of heparosan in biological samples and facilitate pharmaceutical applications.

Main Methods:

  • Utilized the enzyme repertoire of E. coli K5 and bacteriophage ΦK5B for heparosan synthesis and depolymerization.
  • Employed fluorescently labeled heparosan nonamer to study the elongation mechanism of heparosan polymerases KfiA and KfiC.
  • Identified and characterized the heparosan lyase activity of bacteriophage ΦK5B tailspike protein KflB, including its depolymerization mechanism.

Main Results:

  • Demonstrated distributive action of polymerases KfiA and KfiC, producing labeled heparosan with low dispersity.
  • Identified KflB as a heparosan lyase and characterized its endolytic depolymerization mechanism.
  • Engineered an inactive KflB variant for sensitive heparosan detection in ELISA, blots, and cell-based assays.

Conclusions:

  • Enzymatically synthesized heparosan serves as a valuable tool for biochemical studies and detection assays.
  • The developed inactive KflB variant provides a robust method for heparosan detection, overcoming previous limitations.
  • This work advances the utility of heparosan in pharmaceutical research and diagnostics.