Alsobacter ponti sp. nov., a novel denitrification and sulfate reduction bacterium isolated from Pearl River sediment

A Gram-staining negative, aerobic, motile, and short rods strain, designated SYSU M60028^T, was isolated from a Pearl River sediment sample in Guangzhou, Guangdong, China. The isolate could be able to grow at pH 6.0-8.0 (optimum, pH 7.0), 25-37 °C (optimum, 28 °C) and in the presence of 0-2% (w/v) NaCl (optimum, 0% NaCl). The cellular polar lipids of this strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and three unidentified lipids. The respiratory quinone of SYSU M60028^T was found to be Q-10. The major fatty acids (> 5% of total) were summed feature 8, C_16:0, and C_18:1 ω7c 11-methy1. The genomic DNA G + C content was 69.9%. Phylogenetic analyses based on 16S rRNA gene sequences and core genes indicated that strain SYSU M60028^T belonged to the genus Alsobacter and had the highest sequences similarities to Alsobacter metallidurans SK200a-9^T (96.87%) and Alsobacter soli SH9^T (96.87%). Based on the phenotypic, genotypic, and phylogenetic data, strain SYSU M0028^T should be considered to represent a novel species of the genus Alsobacter, for which the name Alsobacter ponti sp. nov. is proposed. The type strain is SYSU M60028^T (= CGMCC 1.19341^T = KCTC 92046^T).