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Antioxidants Amelioration Is Insufficient to Prevent Acrylamide and Alpha-Solanine Synergistic Toxicity in BEAS-2B

Hoda Awad Eltayeb1,2, Leandra Stewart1, Mounira Morgem1,2

  • 1Department of Biology, Texas Southern University, Houston, TX 77004, USA.

International Journal of Molecular Sciences
|August 12, 2023
PubMed

Insights

Antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) did not protect BEAS-2B cells from the combined toxic effects of acrylamide and alpha-solanine. These compounds synergistically inhibited cell signaling pathways.

Area of Science:

  • Cellular toxicology
  • Molecular biology
  • Biochemistry

Background:

  • Free radicals generated during cellular metabolism can damage lipids, proteins, and nucleic acids.
  • Antioxidants neutralize free radicals, preventing cellular damage.
  • Acrylamide and alpha-solanine are toxic compounds found in potato products.

Purpose of the Study:

  • To investigate the protective effects of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) against the synergistic toxicity of acrylamide and alpha-solanine in BEAS-2B cells.
  • To analyze the impact of these compounds on cell morphology, DNA, RNA, and protein expression.

Main Methods:

  • BEAS-2B cells were treated with BHA and BHT, followed by exposure to acrylamide and alpha-solanine.
  • Cell morphology was observed, and analyses of DNA, RNA, and protein were performed.
  • Polymerase chain reaction (PCR) was used to assess gene expression, including AKT/PKB signaling.

Main Results:

  • BHA and BHT did not prevent the synergistic toxic effects of acrylamide and alpha-solanine.
  • Cell morphology was altered, and RNA levels were reduced, but DNA integrity was maintained.
  • Acrylamide and alpha-solanine synergistically inhibited AKT/PKB expression and its downstream targets.

Conclusions:

  • Butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) do not offer protection against the combined toxicity of acrylamide and alpha-solanine in BEAS-2B cells.
  • The synergistic action of these toxicants impacts cellular components and signaling pathways, specifically inhibiting AKT/PKB.
  • Further research is needed to understand the mechanisms of toxicity and explore potential protective strategies.
Keywords:
AKT/PKBBEAS-2B cellsacrylamidealpha (α)-solaninebutylated hydroxyanisole (BHA)butylated hydroxytoluene (BHT)

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