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Triangular Silver Nanoplates as a Bioanalytical Tool: Potential COVID-19 Detection.

Laura G Rodriguez Barroso1, Eduardo Lanzagorta Garcia1, Marija Mojicevic1

  • 1PRISM Research Institute, Technological University of the Shannon: Midlands Midwest, Dublin Rd, N37 HD68 Athlone, Ireland.

International Journal of Molecular Sciences
|August 12, 2023
PubMed
Summary

This study developed enhanced gold-coated silver nanoplates for sensitive Spike protein detection in molecular diagnostics. The nanoplates demonstrated specific binding in complex environments, crucial for accurate biosensing applications.

Keywords:
COVID-19TSNPfibronectinimmunoassayimmunodetectionnanoplatesspike

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Area of Science:

  • Nanotechnology
  • Molecular Diagnostics
  • Biosensors

Background:

  • Nanoparticles offer advanced capabilities for molecular diagnostics.
  • Gold-coated silver nanoplates with PEG coating show promise as biosensor upgrades.

Purpose of the Study:

  • To evaluate PEG-coated gold-coated silver nanoplates (PEGAuTSNPs) for sensitive Spike protein detection.
  • To demonstrate the utility of antibody-coated PEGAuTSNPs in complex biological environments for immunoassays.

Main Methods:

  • Utilized localized surface plasmon resonance (LSPR) and dynamic light scattering (DLS) for analysis.
  • Investigated fibronectin-anti-fibronectin binding in an extracellular matrix model.
  • Determined optimal Spike protein functionalization concentration (4 µg/mL).
  • Employed TJP1 protein as a negative control and horse serum to simulate complex conditions.

Main Results:

  • PEGAuTSNPs demonstrated specific binding of anti-Spike protein.
  • Stronger antibody affinity observed, displacing TJP1 from the nanoplates' surface.
  • Successful detection of specific binding in a simulated complex environment.

Conclusions:

  • Antibody-coated PEGAuTSNPs are effective tools for sensitive immunoassays.
  • Consideration of complex environments and appropriate controls is vital for accurate molecular diagnostics.
  • Further research will focus on optimizing immunoassay performance in diverse complex settings.