Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A single-cell multi-omics assay for simultaneous measurement of vector copy number and protein expression in CAR T cells.

Molecular therapy. Advances·2026
Same author

Intermethod Characterization of Commercially Available Extracellular Vesicles as Reference Materials.

Biomolecules·2026
Same author

Calibration of Flow Cytometers Enables Reproducible Measurements of Extracellular Vesicle Concentrations and Reference Range Establishment.

Journal of extracellular vesicles·2025
Same author

Evaluation of sources of variability in a nitric oxide screening assay for engineered nanomaterials.

Nanomedicine : nanotechnology, biology, and medicine·2025
Same author

AI and flow cytometry.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Gene Copy Number Dictates Extracellular Vesicle Cargo.

International journal of molecular sciences·2025
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jul 19, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.7K

Development of a Cell-Based SARS-CoV-2 Pseudovirus Neutralization Assay Using Imaging and Flow Cytometry Analysis.

Jerilyn R Izac1, Edward J Kwee1, Linhua Tian1

  • 1Biosystems and Biomaterials Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899, USA.

International Journal of Molecular Sciences
|August 12, 2023
PubMed
Summary
This summary is machine-generated.

A new vesicular stomatitis virus (VSV)-based assay rapidly measures neutralizing antibodies against SARS-CoV-2. This method aids in tracking immune response and developing effective COVID-19 treatments.

Keywords:
SARS-CoV-2antibodiesdiagnosticflow cytometryimagingneutralizationpseudovirus

More Related Videos

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
05:49

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

1.7K
Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research
06:08

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

1.3K

Related Experiment Videos

Last Updated: Jul 19, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.7K
Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
05:49

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

1.7K
Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research
06:08

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

1.3K

Area of Science:

  • Virology
  • Immunology
  • Infectious Diseases

Background:

  • The COVID-19 pandemic necessitates accurate measurement of neutralizing antibodies to SARS-CoV-2.
  • Existing methods for assessing antibody neutralization can be slow or complex.
  • Rapid and reliable assays are crucial for vaccine efficacy assessment and therapeutic development.

Purpose of the Study:

  • To develop and validate a rapid, user-friendly neutralization assay for SARS-CoV-2.
  • To quantify functional neutralizing antibodies in patient serum samples.
  • To provide a tool for faster diagnosis and treatment evaluation during pandemics.

Main Methods:

  • Development of a vesicular stomatitis virus (VSV)-based pseudovirus neutralization assay.
  • Utilized two distinct readout methods: live-cell imaging and flow cytometry.
  • Optimized assay parameters including pseudovirus type and multiplicity of infection (MOI).

Main Results:

  • The VSV-based assay successfully quantified varying levels of SARS-CoV-2 neutralization in patient sera.
  • Assay results demonstrated strong correlation with established serological and surrogate neutralization assays.
  • Both imaging and flow cytometry readouts yielded comparable 50% neutralization titer (NT50) values.

Conclusions:

  • The developed VSV-based assay offers a rapid and efficient method for evaluating SARS-CoV-2 neutralizing antibody responses.
  • In situ and high-throughput capabilities enable swift assessment critical for pandemic mitigation.
  • This assay serves as a valuable tool for monitoring vaccine effectiveness and guiding therapeutic strategies.