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Coffea arabica Extract Attenuates Atopic Dermatitis-like Skin Lesions by Regulating NLRP3 Inflammasome Expression and
Qiao-Xin Chang1, Jia-Ling Lyu1,2, Po-Yuan Wu3,4
1Department of Cosmeceutics, China Medical University, Taichung 406, Taiwan.
Abstract:
Atopic dermatitis (AD) is a common skin disease worldwide. The major causes of AD are skin barrier defects, immune dysfunction, and oxidative stress. In this study, we investigated the anti-oxidation and anti-inflammation effects of Coffea arabica extract (CAE) and its regulation of the skin barrier and immune functions in AD. In vitro experiments revealed that CAE decreased the reactive oxygen species levels and inhibited the translocation of nuclear factor-κB (NF-κB), further reducing the secretion of interleukin (IL)-1β and IL-6 induced by interferon-γ (IFN-γ)/tumor necrosis factor-α (TNF-α). Moreover, CAE decreased IFN-γ/TNF-α-induced NLR family pyrin domain-containing 3 (NLRP3), caspase-1, high-mobility group box 1 (HMGB1), and receptor for advanced glycation end products (RAGE) expression levels. It also restored the protein levels of skin barrier function-related markers including filaggrin and claudin-1. In vivo experiments revealed that CAE not only reduced the redness of the backs of mice caused by 2,4-dinitrochlorobenzene (DNCB) but also reduced the levels of pro-inflammatory factors in their skin. CAE also reduced transepidermal water loss (TEWL) and immune cell infiltration in DNCB-treated mice. Overall, CAE exerted anti-oxidation and anti-inflammation effects and ameliorated skin barrier dysfunction, suggesting its potential as an active ingredient for AD treatment.
Insights
Coffea arabica extract (CAE) shows significant anti-oxidation and anti-inflammation effects. It improves skin barrier function and reduces inflammation, suggesting potential for atopic dermatitis (AD) treatment.
Area of Science:
- Dermatology
- Immunology
- Pharmacology
Background:
- Atopic dermatitis (AD) is a prevalent global skin condition.
- Key factors contributing to AD include skin barrier defects, immune system dysfunction, and oxidative stress.
Purpose of the Study:
- To investigate the anti-oxidation and anti-inflammation properties of Coffea arabica extract (CAE).
- To evaluate CAE's effects on skin barrier and immune functions in the context of atopic dermatitis.
Main Methods:
- In vitro assays to assess reactive oxygen species (ROS) levels and inflammatory marker secretion (IL-1β, IL-6).
- Analysis of key inflammatory pathway components (NF-κB, NLRP3, caspase-1, HMGB1, RAGE) and skin barrier proteins (filaggrin, claudin-1).
- In vivo studies using a mouse model of atopic dermatitis induced by 2,4-dinitrochlorobenzene (DNCB).
Main Results:
- CAE reduced ROS levels and inhibited NF-κB translocation, decreasing pro-inflammatory cytokine secretion (IL-1β, IL-6).
- CAE downregulated inflammatory markers (NLRP3, caspase-1, HMGB1, RAGE) and restored skin barrier proteins (filaggrin, claudin-1).
- In vivo, CAE reduced skin redness, inflammation, transepidermal water loss (TEWL), and immune cell infiltration in DNCB-treated mice.
Conclusions:
- Coffea arabica extract demonstrates significant anti-oxidative and anti-inflammatory effects.
- CAE effectively ameliorates skin barrier dysfunction and immune dysregulation associated with atopic dermatitis.
- CAE shows promise as a potential therapeutic ingredient for treating atopic dermatitis.
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