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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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mirMachine: A One-Stop Shop for Plant miRNA Annotation
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miR-430 microRNA Family in Fishes: Molecular Characterization and Evolution.

Claudio A Jiménez-Ruiz1, Roberto de la Herrán1, Francisca Robles1

  • 1Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Avda. Fuentenueva s/n, 18071 Granada, Spain.

Animals : an Open Access Journal From MDPI
|August 12, 2023
PubMed
Summary

The miR-430 microRNA family in fish plays roles in early development and metamorphosis. This study reveals its evolutionary history, identifying three variants and suggesting DNA rearrangements impact its organization.

Keywords:
genomic organizationnoncoding RNAphylogenetic and evolutionary analysistandem repeats

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Area of Science:

  • Developmental Biology
  • Genomics
  • Evolutionary Biology

Background:

  • The miR-430 microRNA family is among the first expressed by fish zygotes.
  • It is potentially involved in maternal mRNA elimination, steroidogenesis, sexual differentiation, and flatfish metamorphosis.
  • miR-430 sequences are typically found in tandem clusters, with limited evidence of conservation outside teleost fishes.

Purpose of the Study:

  • To characterize the tandem repeat organization of the miR-430 microRNA family in diverse fish species.
  • To investigate the evolutionary history and conservation of the miR-430 family.
  • To understand the variability and organization of miR-430 in fish.

Main Methods:

  • Tandem repeat organization characterization in various fish species.
  • Phylogenetic analysis of the miR-430 family.
  • Detection of miR-430 repeat subunits.

Main Results:

  • The miR-430 family originated from a duplication event predating the divergence of Chondrostei and Neopterygii, yielding three variants (a, b, c).
  • Variant 'b' is the most closely related to the ancestral miR-430 sequence.
  • Isolated miR-430 repeat subunits were found in some species, indicating potential DNA rearrangements.

Conclusions:

  • The miR-430 family exhibits significant variability and complex organization in fish.
  • Phylogenetic analysis clarifies the evolutionary relationships and duplication events within the miR-430 family.
  • The findings contribute new insights into the abundance, variability, and genomic organization of fish microRNAs.