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The Small RNA Component of Arabidopsis thaliana Phloem Sap and Its Response to Iron Deficiency
Ahmet Bakirbas1, Rosario Castro-Rodriguez2, Elsbeth L Walker2
1Biology Department and Plant Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, USA.
Abstract:
In order to discover sRNA that might function during iron deficiency stress, RNA was prepared from phloem exudates of Arabidopsis thaliana, and used for RNA-seq. Bioanalyzer results indicate that abundant RNA from phloem is small in size-less than 200 nt. Moreover, typical rRNA bands were not observed. Sequencing of eight independent phloem RNA samples indicated that tRNA-derived fragments, specifically 5' tRFs and 5' tRNA halves, are highly abundant in phloem sap, comprising about 46% of all reads. In addition, a set of miRNAs that are present in phloem sap was defined, and several miRNAs and sRNAs were identified that are differentially expressed during iron deficiency.
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