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A fluorometric high performance liquid chromatographic assay for vanillylmandelic acid (VMA) developed from a
Abstract:
A fluorometric procedure based on the Bio-Rad Vanillylmandelic Acid (VMA) by High Performance Liquid Chromatography (HPLC) Test was developed. Detection by fluorescence provides better sensitivity and specificity than the Pisano spectrophotometric method. Use of a C18 mini-column extraction to extract VMA prior to HPLC analysis significantly reduced the late eluting peaks. A linear range was established up to 400 mumol/L of VMA. Within-run and between-run CV's were 3.0% and 3.7%, respectively. In a comparison of this fluorometric method with the Pisano spectrophotometric method, a linear regression of y = 0.807x + 2.185 was obtained with a correlation coefficient of 0.945. In the analysis of over 50 samples, no intereferences have been found.