Structural Characterization of DNA-Templated Silver Nanoclusters by Energy Dispersive Spectroscopy

Here, a novel method of structural determination for DNA-templated silver nanoclusters (DNA-AgNCs) is introduced. This technique uses energy dispersive spectroscopy (EDS) coupled with a scanning electron microscope (SEM) to analyze a monodisperse solution of nucleic acid-based structures. Exploiting the consistent number of phosphate atoms in each structure, we determine the average number of silver atoms that make up the DNA-AgNCs. Proper sample preparation and fine-tuning of the SEM/EDS system settings were combined to achieve highly repeatable data.