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Updated: Jul 19, 2025
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
Published on: April 29, 2014
MSC.sensor: Capturing cancer cell interactions with stroma for functional profiling
Yun Huang1, Aneta Drakul2, Jasmeet Sidhu3
1Division of Oncology and Children's Research Centre, University Children's Hospital Zurich, Zurich, Switzerland; Moores Cancer Center, University of California, San Diego, La Jolla, CA, United States.
None:
Mesenchymal stromal cells (MSCs) contribute to the microenvironment regulating normal and malignant hematopoiesis, and thus may support subpopulations of cancer cells to escape therapeutic pressure. Here, we engineered bone marrow MSCs to express a synthetic CD19-sensor receptor to detect and display interacting primary CD19+ leukemia cells in coculture. This implementation provides a versatile platform facilitating ex vivo drug response profiling of primary CD19+ leukemia cells in coculture with high-sensitivity and scalability.
