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Using Cu2+ to regulate the emission feature of near-infrared fluorescent sensor with AIE: To detect ascorbic acid in
Qian Meng1, Junxiong Yao1, Mengyu Chen1
1School of Chemistry and Materials Engineering, Engineering Research Center of Biomass Conversion and Pollution Prevention of Anhui Educational Institutions, Anhui Provincical Key Laboratory for Degradation and Monitoring of Pollution of the Environment, Fuyang Normal University, Fuyang, Anhui Province, 236037, PR China.
Abstract:
Conventional ascorbic acid (AA) detection methods such as chromatography, capillary electrophoresis, colorimetry, electrochemical detection, and enzymatic analysis require expensive equipment and complicated operation. Simple, rapid, and accurate AA detection is essential to inspect food quality, diagnose diseases, and assess immunity in humans. In this study, the first near-infrared fluorescence sensor DBHM with aggregation-induced emission was developed to detect AA under the involvement of Cu2+. The DBHM + Cu2+ sensor showed high sensitivity to AA with a limit of detection of 2.37 μM. The AA detection mechanism was investigated by optical studies, 1H NMR titration, high-resolution mass spectrometry, and infrared spectroscopy. AA was detected qualitatively and quantitatively by the DBHM + Cu2+ sensor in beverages, fruits, and Vitamin C tablets using a dual-mode (fluorescence and smartphone app) sensing platform. The new sensing system also showed low toxicity and excellent bioimaging in HeLa cells, C. elegans, and mice. This sensor could advance AA detection technology in the food industry and has potential bioimaging applications.
