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3D DNAzyme walker based electrochemical biosensor for attomolar level microRNA-155 detection
Jiaying Zhao1, Congjuan He1, Yanyi Long1
1Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Analytica Chimica Acta
|August 12, 2023
Summary
This study presents a novel biosensor for detecting microRNA-155 (miR-155) using a 3D DNAzyme walker and a flexible paper-based electrode. The biosensor achieves ultrasensitive detection with a low limit of detection, showing promise for clinical diagnostics.
Area of Science:
- * Biomedical Engineering
- * Analytical Chemistry
- * Molecular Diagnostics
Background:
- * Accurate detection of microRNAs (miRNAs) is crucial for early disease diagnosis.
- * Existing methods for miRNA detection often face challenges with sensitivity and complexity.
- * Development of novel biosensors is needed for improved clinical disease diagnosis.
Purpose of the Study:
- * To construct an ultrasensitive electrochemical biosensor for microRNA-155 (miR-155) detection.
- * To utilize a 3D DNAzyme walker for signal amplification.
- * To employ a gold nanoparticles/graphene aerogels carbon fiber paper-based (AuNPs/GAs/CFP) flexible sensing electrode.
Main Methods:
- * Construction of a 3D DNAzyme walker system with magnetic beads (MBs) and Fe-MOF-NH2 nanoparticles.
- * Development of a paper-based flexible sensing electrode (AuNPs/GAs/CFP).
- * Signal amplification through a recognition-catalysis-release cycle driven by the DNAzyme walker.
Main Results:
- * The biosensor achieved ultrasensitive detection of miR-155 with a low limit of detection (LOD) of 56.23 aM.
- * A wide linear detection range from 100 aM to 100 nM was established.
- * The biosensor demonstrated good interference resistance and reproducibility.
Conclusions:
- * The developed 3D DNAzyme walker biosensor provides a highly sensitive and reliable platform for miR-155 detection.
- * The flexible paper-based electrode offers advantages for potential point-of-care applications.
- * This approach shows significant potential for advancing clinical disease diagnosis through sensitive miRNA detection.
