Long noncoding RNA XLOC_006786 inhibits the proliferation, invasion and metastasis of osteosarcoma cells through NOTCH3 signaling pathway by targeting miR-491-5p

Area of Science:

• Molecular Biology
• Oncology
• Genetics

Background:

• Long noncoding RNAs (LncRNAs) play significant roles in various diseases, including cancers.
• The specific function of LncRNA XLOC_006786 in human osteosarcoma remains largely uncharacterized.

Purpose of the Study:

• To elucidate the role and mechanism of LncRNA XLOC_006786 in human osteosarcoma.
• To investigate its potential as a tumor suppressor and therapeutic target.

Main Methods:

• Quantitative reverse transcription PCR (RT-qPCR) and Western blotting were used to assess gene and protein expression.
• Cell Counting Kit-8 (CCK-8) and Transwell assays evaluated cell proliferation, invasion, and migration.
• In vivo experiments were conducted to assess the effect on lung metastasis.

Main Results:

• LncRNA XLOC_006786 significantly inhibited osteosarcoma cell proliferation, invasion, and migration.
• LncRNA XLOC_006786 was found to negatively regulate the oncogene NOTCH3.
• Bioinformatics and experimental validation confirmed LncRNA XLOC_006786 targets miR-491-5p, which in turn targets NOTCH3, suppressing tumor growth and metastasis in vivo.

Conclusions:

• LncRNA XLOC_006786 functions as a tumor suppressor in osteosarcoma.
• It exerts its effects by inhibiting proliferation, invasion, and metastasis through the miR-491-5p/NOTCH3 signaling pathway.