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Updated: Jul 19, 2025

A Method for Characterizing Embryogenesis in Arabidopsis
Published on: August 4, 2017
Arabidopsis NOTCHLESS plays an important role in root and embryo development
Ke Li1, Qingtian Zhang1, Huiping Liu1
1Shandong Academy of Grape, Shandong Academy of Agricultural Sciences, Jinan, PR China.
Abstract:
Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis (A. thaliana) remains exclusive. Here, we found that Arabidopsis NLE (AtNLE) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development.
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