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Sustainable metabolic engineering requires a perfect trifecta
Glenn Nurwono1, Samantha O'Keeffe2, Nian Liu2
1Department of Chemistry and Biochemistry, USA.
Current Opinion in Biotechnology
|August 13, 2023
Summary
Metabolic engineering offers sustainable chemical alternatives by optimizing cellular metabolism. A reverse engineering approach guides selecting the ideal substrate, product, and organism for efficient bioprocess development.
Area of Science:
- Biotechnology and metabolic engineering
- Sustainable chemical synthesis
- Industrial microbiology
Background:
- Cellular metabolism provides versatile routes for sustainable chemical production.
- Metabolic engineering can enhance product yield, rate, and titer.
- Navigating substrate-product-organism combinations is complex for metabolic engineering.
Purpose of the Study:
- To propose a reverse engineering strategy for metabolic engineering.
- To guide the selection of substrate, product, and organism for sustainable bioprocesses.
- To address challenges in navigating metabolic engineering project parameters.
Main Methods:
- Reverse engineering strategy initiated with product selection.
- Subsequent pairing of substrate and organism based on product requirements.
- Analysis of existing bioproduct synthesis using conventional and unconventional feedstocks.
Main Results:
- A significant bioproduct space has been explored.
- Top compounds are primarily synthesized using glucose and model organisms.
- Unconventional feedstocks and non-model organisms show promise for advanced bioproduct synthesis.
Conclusions:
- A perfect substrate-organism-product trifecta is crucial for sustainable metabolic engineering.
- Judicious selection illuminates untapped potential in metabolic engineering.
- The proposed strategy aids in navigating complex metabolic engineering endeavors.
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