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Updated: Jul 19, 2025
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Polyvalent aptamer scaffold coordinating light-responsive oxidase-like nanozyme for sensitive detection of
Ziyang He1, Jinxin Zhang1, Mei Liu2
1College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
Abstract:
An efficient aptasensor was developed for the colorimetric determination of zearalenone (ZEN) based on polyvalent aptamer scaffold and light-responsive oxidase-like nanozyme. The sensitivity and efficiency of the development method were significantly improved owing to rich aptamers and signal labels (3, 4-dihydroxybenzoic acid, PCA) packed in the scaffold. The scaffold integrated functions of target recognition, surface immobilization and signal transduction. The photoresponsive nanoenzyme of TiO2-PCA was formed by PCA coordinated with Ti (IV) on the surface of TiO2. TiO2-PCA catalyzed dissolved oxygen rather than H2O2 to generate colorimetric signal by stimulating the chromogenic substrate, which made the assay greener and safer. The detection limit of colorimetric mode was 0.0087 ng/mL and the satisfactory recoveries 92.00 %-111.00 % were achieved in spiked food samples. This strategy opens new horizons for sensitive detection of small molecule hazards and promises to be a powerful tool for safeguarding food safety.
