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Published on: September 30, 2016
Tumorigenesis mechanism and application strategy of the MDCK cell line: A systematic review
Di Yang1, Lingwei Huang2, Jiamin Wang2
1Engineering Research Center of Key Technology and Industrialization of Cell-based Vaccine, Ministry of Education, Biomedical Research Center, Northwest Minzu University, Lanzhou, 730030, China; Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, 730030, Lanzhou, China; College of Veterinary Medicine, Gansu Agricultural University, 730030, Lanzhou, China; Department of Experiment & Teaching, Northwest Minzu University, 730030, Lanzhou, China.
Abstract:
Influenza is an acute respiratory infectious disease caused by influenza virus that seriously endangers people's health. Influenza vaccination is the most effective means to prevent influenza virus infection and its serious complications. MDCK cells are considered to be superior to chicken embryos for the production of influenza vaccines, but the tumorigenicity of cells is a concern over the theoretical possibility of the risk of adverse events. The theoretical risks need to be adequately addressed if public confidence in programs of immunization are to be maintained. In this paper, studies of the tumorigenic potential of cell lines, with MDCK cells as an example, published since 2010 are reviewed. The mechanism of tumorigenicity of MDCK cells is discussed with reference to cell heterogeneity and epithelial to mesenchymal transition (EMT). Understanding the mechanism of the acquisition of a tumorigenic phenotype by MDCK cells might assist in estimating potential risks associated with using tumorigenic cell substrates for vaccine production.
Insights
Influenza vaccines are crucial for public health. This review examines the tumorigenic potential of Madin Darby Canine Kidney (MDCK) cells used in vaccine production, addressing safety concerns.
Area of Science:
- Virology and Immunology
- Cell Biology
- Vaccine Development
Background:
- Influenza poses a significant global health threat, necessitating effective prevention strategies like vaccination.
- Influenza vaccines are primarily produced using chicken embryos or Madin Darby Canine Kidney (MDCK) cells.
- MDCK cells offer advantages for vaccine production, but their potential tumorigenicity raises safety concerns.
Approach:
- This paper reviews studies published since 2010 focusing on the tumorigenic potential of cell lines, specifically MDCK cells.
- The review analyzes the mechanisms underlying MDCK cell tumorigenicity, including cell heterogeneity and epithelial to mesenchymal transition (EMT).
Key Points:
- MDCK cells are increasingly used for influenza vaccine manufacturing.
- The tumorigenicity of MDCK cells is a theoretical concern for vaccine safety.
- Cell heterogeneity and EMT are implicated in the acquisition of tumorigenic phenotypes by MDCK cells.
Conclusions:
- Understanding MDCK cell tumorigenicity mechanisms is vital for assessing risks in vaccine production.
- Addressing theoretical risks associated with cell substrates is crucial for maintaining public confidence in immunization programs.
- Further research into cell substrate safety is essential for advancing vaccine technology.
