Delignification of the cotton stalk and ginning mill waste via EnZolv pretreatment and optimization of process
Santhoshkumar Subramaniam1, Kumutha Karunanandham2, Raja Asm3
1Biocatalysts Laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University (TNAU), Coimbatore 641003, Tamil Nadu, India; Department of Agricultural Microbiology, Agricultural College and Research Institute, Madurai 625104, Tamil Nadu, India.
Abstract:
The present study aimed to add value to cotton waste biomass using a more eco-friendly process, EnZolv which delignifies cotton stalk and cotton ginning mill waste. A maximum delignification of 68.68% and 65.51% was obtained using pre-optimized EnZolv parameters in cotton stalk (CS) and ginning mill waste (GMW), respectively. Optimized EnZolv process removed 78.68% of lignin in CS using Response Surface Methodology (RSM) in Box-Behnken design at 0% moisture content, 50 U laccase g-1 of biomass, 5 h incubation time, 50 ⁰C incubation temperature, and 150 rpm shaking speed. Similarly, RSM-based delignification of 70.53% in GMW was achieved under the optimized EnZolv conditions of 98.75 % moisture content, 41.59 U laccase g-1 of biomass, 9.3 h incubation time, 46.15 ⁰C incubation temperature, and 150 rpm shaking speed.
