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Hatchability evaluation of bovine IVF embryos using OCT-based 3D image analysis.

Yasumitsu Masuda1, Ryo Hasebe2, Yasushi Kuromi2

  • 1Department of Animal Science, Tottori Livestock Research Center, Tottori 680-8553, Japan.

The Journal of Reproduction and Development
|August 13, 2023
PubMed
Summary

Optical coherence tomography (OCT) can predict bovine embryo quality. OCT-assessed structural data, like trophectoderm thickness and blastocoel volume, correlate with in vitro hatchability in cattle embryos.

Keywords:
Bovine embryoHatchingIVFOptical coherence tomographyQuantification of embryo structure

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Area of Science:

  • Reproductive biology
  • Biotechnology
  • Veterinary medicine

Background:

  • Embryo transfer in cattle often fails to establish pregnancy.
  • Accurate evaluation of bovine embryo quality is crucial for successful in vitro fertilization (IVF) and transfer outcomes.
  • Novel parameters are needed to improve bovine embryo assessment.

Purpose of the Study:

  • To investigate the relationship between in vitro hatchability and morphological parameters of bovine embryos using optical coherence tomography (OCT).
  • To identify new parameters for evaluating bovine embryo quality and predicting hatchability.
  • To establish OCT as a tool for assessing structural data of blastocyst-stage embryos.

Main Methods:

  • Bovine embryos from Japanese Black cattle were produced via IVF.
  • Blastocyst quality was assessed using inverted microscopy (IETS Codes 1-3).
  • Three-dimensional OCT imaging was performed on Day 7 post-IVF, followed by hatchability assessment on Day 9. 22 parameters were measured for 42 embryos.

Main Results:

  • Hatched (H) embryos exhibited significantly lesser trophectoderm (TE) and TE+zona pellucida (ZP) thickness compared to non-hatched (NH) embryos.
  • H embryos showed greater volumes of TE, ZP, blastocoel, and the whole embryo, along with a larger blastocoel diameter.
  • Principal Component Analysis (PCA) indicated that increased blastocoel-related values and decreased TE/ZP thickness are indicators of hatchability.

Conclusions:

  • OCT-derived structural parameters can serve as potential predictors of bovine IVF embryo quality and hatchability.
  • Morphological features assessed by OCT, such as TE thickness and blastocoel volume, are linked to successful embryo development.
  • This study supports the use of OCT imaging for non-invasive, quantitative evaluation of bovine embryos, potentially improving pregnancy rates.