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Effective assessment of lanthanide ion delivery into live cells by paramagnetic NMR spectroscopy
Jia-Liang Chen1,2, Yin Yang2, Tiesheng Shi1
1College of Chemistry, Chemical Engineering and Materials Science, Zaozhuang University, Zaozhuang, Shandong, 277160, China. 819093@nankai.edu.cn.
Abstract:
We report an effective assessment of lanthanide ion (Ln3+) delivery into live cells by paramagnetic NMR spectroscopy. Free Ln3+ ions are toxic to live cells resulting in a gradual leakage of target proteins to the extracellular media. The citrate-Ln3+ complex is an efficient and mild reagent over the free Ln3+ form for live cell delivery.

