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An end-to-end workflow for non-destructive 3D pathology.

Kevin W Bishop1,2, Lindsey A Erion Barner1, Qinghua Han1,2

  • 1Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA.

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Summary

This study presents a comprehensive protocol for generating high-quality 3D pathology datasets. It details a workflow for volumetric tissue imaging, crucial for advancing preclinical and clinical research.

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Area of Science:

  • Pathology
  • Microscopy
  • Biotechnology

Background:

  • Conventional 2D pathology lacks volumetric context.
  • 3D pathology enables imaging larger tissue volumes without destruction.
  • Consistent generation of high-quality 3D pathology data is challenging.

Approach:

  • An end-to-end protocol for 3D pathology workflow is provided.
  • Light-sheet microscopy is used as an illustrative imaging platform.
  • Focuses on a fluorescent analog of hematoxylin and eosin (H&E) staining.

Key Points:

  • The protocol covers tissue preparation, imaging, and data processing.
  • It facilitates well-controlled preclinical and clinical studies.
  • Guidelines are presented in a tutorial format for diverse end-users.

Conclusions:

  • This protocol standardizes 3D pathology workflows.
  • It enhances volumetric tissue analysis capabilities.
  • Aimed at biologists, clinical researchers, and engineers.