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Flow Cytometric Analysis of Regulated Cell Death.
1Department of Trauma Medical Center, State Key Laboratory of Trauma, Burns and Combined Injury, Daping Hospital, Army Medical University, Chongqing, China.
Methods in Molecular Biology (Clifton, N.J.)
|August 14, 2023
Summary
Flow cytometry is essential for quantifying regulated cell death processes like apoptosis and pyroptosis. This guide offers methods for analyzing cell death dynamics in mammalian cells.
Area of Science:
- Cellular Biology
- Molecular Biology
- Physiology
Background:
- Cell death is a fundamental biological process vital for development and homeostasis.
- Understanding diverse cell death mechanisms, including apoptosis, autophagy, ferroptosis, pyroptosis, and immunogenic cell death, is crucial.
- Quantitative analysis of cell death dynamics is increasingly necessary.
Purpose of the Study:
- To provide guidelines for using flow cytometry to detect and quantify regulated cell death.
- To offer a comprehensive, high-throughput method for analyzing cell death at the single-cell level.
Main Methods:
- Flow cytometry is presented as the primary method for cell death detection.
- Guidelines cover the application of flow cytometry to various regulated cell death types.
Main Results:
- Flow cytometry enables high-throughput, single-cell level quantification of cell death.
- The method is applicable to a range of regulated cell death pathways.
Conclusions:
- Flow cytometry is a versatile and essential tool for studying regulated cell death.
- Standardized guidelines facilitate accurate and reproducible cell death analysis.
