Related Experiment Video
Updated: Jul 19, 2025

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
Published on: June 14, 2018
Knockout of a gene encoding a Gγ protein boosts alkaline tolerance in cereal crops
1Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046 Japan.
Abstract:
Sorghum is highly tolerant to alkaline stress, but the underlying mechanisms are not well understood. Here, based on genotypic difference in alkaline tolerance of sorghum, it was found that AT1 (Alkaline tolerance 1) encoding a G protein is involved in alkaline tolerance through negatively modulating the phosphorylation level of PIP2, an aquaporin with transport activity for H2O2. Knockout of AT1 releases its inhibition of PIP2, thereby resulting in an increased transport of H2O2 from the cytosol into the apoplast, subsequently boosting alkaline tolerance.
More Related Videos
Related Concept Videos
In-vitro Mutagenesis
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Breeding and Biotechnology

