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A lipid droplet-associated protein Nem1 regulates appressorium function for infection of Magnaporthe oryzae

Deng Chen1, Xuan Cai1, Junjie Xing2

  • 1State Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.

Abiotech
|August 15, 2023
PubMed

Insights

This study identifies Nem1, a key protein regulating lipid droplet formation and use in the rice blast fungus. Nem1 is crucial for fungal development, infection, and lipid metabolism, offering insights into plant pathogenic fungi.

Area of Science:

  • Mycology
  • Plant Pathology
  • Molecular Biology

Background:

  • Lipid droplets are vital energy stores in fungal conidia for plant pathogen infection.
  • The regulation of lipid droplet formation and utilization in fungi remains poorly understood.

Purpose of the Study:

  • To investigate the role of lipid droplet-associated protein Nem1 in the rice blast fungus, *Magnaporthe oryzae*.
  • To elucidate the regulatory mechanisms of lipid droplet biogenesis and metabolism during fungal development and infection.

Main Methods:

  • Gene deletion and characterization of *NEM1* in *Magnaporthe oryzae*.
  • Analysis of lipid droplet biogenesis, lipid content (DAG, TAG), asexual development, and virulence.
  • Investigation of Nem1 regulation by TOR and cAMP-PKA signaling pathways and its involvement in autophagy.

Main Results:

  • Nem1 is localized to lipid droplets and crucial for their biogenesis and lipid content.
  • Deletion of *NEM1* impairs conidia production, appressorial penetration, and virulence.
  • Nem1 function is regulated by TOR and cAMP-PKA signaling pathways and linked to autophagy.

Conclusions:

  • Nem1 plays a critical role in regulating lipid metabolism and droplet dynamics in *Magnaporthe oryzae*.
  • Understanding Nem1's function provides insights into fungal development, pathogenicity, and potential targets for disease control.
Keywords:
AppressoriumConidium formationLipid biogenesisMagnaporthe oryzaeTOR signalingcAMP-PKA signaling

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