Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

79
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
79
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

12.0K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.0K
RNA Stability01:53

RNA Stability

33.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.7K
Riboswitches01:56

Riboswitches

8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A diagnostic nomogram for synchronous lung metastasis at initial diagnosis in osteosarcoma: A retrospective SEER database study.

Journal of orthopaedic surgery (Hong Kong)·2026
Same author

Comparison of the Diagnostic Performance of Culture, Histopathology, and mNGS for Periprosthetic Joint Infection Using Periprosthetic Tissue Samples: A Prospective Clinical Study.

Infection and drug resistance·2025
Same author

Blood Cell Ratio Combinations for Diagnosing Periprosthetic Joint Infections: A Preliminary Study.

Infection and drug resistance·2025
Same author

Construction of ferroptosis-related gene signatures for identifying potential biomarkers and immune cell infiltration in osteoarthritis.

Artificial cells, nanomedicine, and biotechnology·2024
Same author

The Arabidopsis Mitochondrial Pseudouridine Synthase Homolog FCS1 Plays Critical Roles in Plant Development.

Plant & cell physiology·2022
Same author

The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata.

The Plant cell·2022
Same journal

The primary beta-galactosidase BGAL10 modulates pavement cell shape acquisition in Arabidopsis.

Journal of experimental botany·2026
Same journal

The link between phosphate starvation-triggered anthocyanin biosynthesis and jasmonate-driven regulation in tomato.

Journal of experimental botany·2026
Same journal

OsFLZ5 Enhances Drought Tolerance and ABA Sensitivity in Rice via Transcriptional Activation by OsbZIP23.

Journal of experimental botany·2026
Same journal

From signaling to catabolism: terminal tails in plant hormone regulation.

Journal of experimental botany·2026
Same journal

Beyond the CO-FT regulatory module: E1 and PHYA emerge as players in photoperiodic regulation of flowering in legumes.

Journal of experimental botany·2026
Same journal

Sulfur metabolism-dependent retrograde signalling for oxidative stress acclimation.

Journal of experimental botany·2026
See all related articles

Related Experiment Video

Updated: Jul 19, 2025

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
06:38

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Published on: February 24, 2021

5.3K

RNA pseudouridine modification in plants.

Yanli Niu1, Lingyun Liu2

  • 1Laboratory of Cell Signal Transduction, School of Basic Medical Sciences, Henan University, Kaifeng 475001, China.

Journal of Experimental Botany
|August 15, 2023
PubMed
Summary
This summary is machine-generated.

Pseudouridine, a key RNA modification, plays a vital role in plant cellular processes. This review explores pseudouridine synthesis, regulation, and function in plants, highlighting its significance beyond yeast and human models.

Keywords:
EpitranscriptomicsRNA modificationsnext-generation sequencingpseudouridinepseudouridine synthases (PUSs)snoRNA

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.7K
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K

Related Experiment Videos

Last Updated: Jul 19, 2025

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
06:38

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Published on: February 24, 2021

5.3K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.7K
A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

2.6K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Plant Science

Background:

  • Pseudouridine is a prevalent RNA modification impacting cellular functions.
  • While extensively studied in yeast and humans, its role in plants is increasingly recognized.
  • Understanding pseudouridine's dynamics is crucial for deciphering RNA regulation.

Approach:

  • This review synthesizes current research on pseudouridine in plant RNA.
  • It covers the synthesis, degradation, and regulatory mechanisms of pseudouridine.
  • Functional roles and future research directions are discussed.

Key Points:

  • Pseudouridine modification is dynamic and essential in plants.
  • Plant pseudouridine landscape influences diverse cellular activities.
  • Evidence highlights critical roles in plant development and stress response.

Conclusions:

  • Pseudouridine is a critical post-transcriptional modification in plants.
  • Further research is needed to fully elucidate its functions and regulatory networks.
  • Investigating pseudouridine offers new avenues for plant biology and biotechnology.