Related Experiment Video
Updated: Jul 19, 2025
08:16
Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms
Published on: March 1, 2022
6.2K
Can endophytic microbial compositions in cane roots be shaped by different propagation methods
Da Yang1, Xinru Lin1, Yufei Wei1
1Guangxi Key Laboratory of Agro-Environment and Agro-Products Safety, National Demonstration Center for Experimental Plant Science Education, Agricultural College, Guangxi University, Nanning, China.
Plos One
|August 15, 2023
Summary
Tissue culture sugarcane seedlings harbor distinct root microbial communities, enhancing plant health and stress resistance compared to traditional stem propagation methods. This study reveals significant differences in bacterial and fungal populations, benefiting sugarcane cultivation.
Area of Science:
- Microbiology
- Plant Science
- Agricultural Science
Background:
- Traditional sugarcane propagation uses stem cuttings, which can lead to disease susceptibility and reduced crop quality.
- Tissue culture offers an alternative propagation method, but its impact on sugarcane root endophytic microbial communities and overall health is not well-understood.
Purpose of the Study:
- To investigate and compare the endophytic microbial compositions in sugarcane roots derived from tissue culture versus traditional stem propagation.
- To evaluate the potential health and stress resistance benefits associated with different sugarcane propagation methods.
Main Methods:
- High-throughput sequencing techniques were employed to analyze the bacterial and fungal endophytic communities in sugarcane roots.
- Root samples were collected from sugarcane grown via tissue culture and stem cuttings.
Main Results:
- Significant differences in endophytic microbial community composition were observed between tissue culture and stem-propagated sugarcane roots.
- Tissue culture seedlings exhibited a higher abundance and diversity of bacterial and fungal operational taxonomic units (OTUs).
- Specific bacterial genera (e.g., Pantoea, Bacillus) and fungal genera (e.g., Rhizoctonia, Fusarium) were enriched in tissue culture-derived roots.
Conclusions:
- Sugarcane propagation methods significantly shape the endophytic microbial communities in the roots.
- Tissue culture propagation appears to enhance the endophytic microbial diversity and composition, potentially improving sugarcane's stress resistance and overall health.
