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Updated: Jul 19, 2025

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
Published on: April 27, 2018
Extramitochondrial ATP as [Ca2+]m and cardiolipin content regulator
Lidiya G Babich1, Sergiy G Shlykov1, Anastasia O Bavelska-Somak1
1Department of Muscle Biochemistry, Palladin Institute of Biochemistry, NAS of Ukraine, 9, Leontovich Str., Kyiv 01054, Ukraine.
Abstract:
An ATP-induced increase of [Ca2+]m in myometrium mitochondria matrix at the absence of exogenous Ca2+ was shown. An ATP-induced increase of Сa2+ efflux from mitochondria ([Сa2+]o) has also been shown. Mitochondria membranes were polarized upon incubation in both Mg2+- and Mg2+,ATP-medium. Cardiolipin (CL) content in mitochondria membranes decreased upon incubation of organelles in Mg2+,ATP-medium as compared to Mg2+-medium. It was suggested that ATP could play the role of a signaling molecule regulating the Ca2+ exchange in the mitochondria.
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