Related Experiment Video
Updated: Jul 19, 2025
12:04
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
9.6K
Mechanistic model for nuclear migration in hyphae during mitosis
1Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700032, India.
Physical Review. E
|August 16, 2023
Summary
Nuclear migration in pathogenic fungi like Saccharomyces cerevisiae and Candida albicans requires robust dynein pulling forces on microtubules. This ensures timely mitosis during hyphal growth, crucial for fungal cell cycle progression.
Area of Science:
- Cell Biology
- Mycology
- Biophysics
Background:
- Saccharomyces cerevisiae and Candida albicans exhibit yeast, pseudohyphae, and true hyphae morphologies.
- True hyphae present unique challenges for nuclear division due to elongated cell structure and variable septin ring localization.
Purpose of the Study:
- To investigate the mechanism of nuclear migration within fungal hyphae.
- To understand how mitosis is executed efficiently in elongated hyphal structures.
Main Methods:
- Exploration of mechanical interactions between astral microtubules and the cell cortex.
- Computational simulation of nuclear migration dynamics.
Main Results:
- Effective nuclear migration requires substantial dynein-mediated pulling forces on astral microtubules.
- Microtubule sliding along the hyphal cortex maximizes dynein engagement for robust pulling.
- Simulation indicates timely migration occurs when dyneins from the mother cortex are disengaged.
Conclusions:
- Dynein-microtubule interactions are critical for accurate nuclear positioning in fungal hyphae.
- The mechanism ensures timely mitosis and nuclear division at the septin ring in elongated hyphal cells.
Related Concept Videos
01:32Cytoskeletal Coordination in Cell Migration
4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K
01:18Role of Myosin in Cell Migration
2.3K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.3K
01:43The Movement of Organelles and Vesicles
4.6K
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.6K
02:11Forces Acting on Chromosomes
3.4K
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
3.4K
02:15M-Cdk Drives Transition Into Mitosis
5.6K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.6K
02:13Determining the Plane of Cell Division
3.4K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function.
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
3.4K