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Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
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Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

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Malignant mesothelioma cells with characteristic intracytoplasmic vacuolization and lipids

Satoshi Morito1,2, Hiroshi Yasui1, Tomoo Itoh3

  • 1Department of Diagnostic Pathology, Rakuwakai Otowa Hospital, Kyoto, Japan.

Diagnostic Cytopathology
|August 16, 2023
PubMed

Insights

This study details rare cytomorphological findings in malignant mesothelioma (MM) cells within pleural effusions. Key features include cytoplasmic vacuolization and specific protein expressions aiding diagnosis.

Area of Science:

  • Cytopathology
  • Oncology
  • Biomarker Discovery

Background:

  • Malignant mesothelioma (MM) diagnosis relies on cytomorphology and immunocytochemistry.
  • Uncommon cytomorphological features can pose diagnostic challenges.

Purpose of the Study:

  • To describe and illustrate unusual cytomorphological characteristics of malignant mesothelioma cells in pleural effusions.
  • To correlate these features with immunocytochemical findings for diagnostic clarity.

Main Methods:

  • Analysis of Giemsa-stained pleural effusion smears.
  • Immunocytochemical staining for calretinin, podoplanin, EMA, MTAP, BRCA1-associated protein 1, CD68, desmin, and adipophilin.

Main Results:

  • Observed abundant cytoplasmic vacuolization and eccentric nuclei in MM cells.
  • Identified a glossy spherical material positive with Sudan III stain.
  • Confirmed MM cells positive for calretinin, podoplanin, EMA, MTAP, and adipophilin.
  • Confirmed MM cells negative for BRCA1-associated protein 1, CD68, and desmin.

Conclusions:

  • Cytoplasmic vacuolization and adipophilin expression are notable features of MM.
  • These cytomorphological and immunocytochemical findings enhance diagnostic accuracy for malignant mesothelioma.
Keywords:
cytologyfoamy cellslipidsmalignant mesotheliomapleural effusion

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