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Fibroblast growth factor 2 acts as an upstream regulator of inhibition of pulmonary fibroblast activation

Xiangqin Tian1, Yangyang Jia1, Yonglong Guo2

  • 1Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, China.

FEBS Open Bio
|August 16, 2023
PubMed

Insights

Fibroblast growth factor 2 (FGF2) inhibits pulmonary fibrosis by suppressing fibroblast activation. FGF2 regulates extracellular matrix organization and fibroblast proliferation, offering a potential therapeutic target for lung fibrosis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Pulmonary Medicine

Background:

  • Fibroblast growth factor (FGF) signaling is vital for lung development and repair.
  • Fibroblast growth factor 2 (FGF2) shows potential in inhibiting pulmonary fibrosis by suppressing fibroblast differentiation.
  • Understanding FGF2's molecular mechanisms in pulmonary fibroblasts (PFs) is crucial.

Purpose of the Study:

  • To elucidate the molecular mechanisms of FGF2-mediated regulation in PFs.
  • To identify genes and biological functions regulated by FGF2 in PFs.
  • To investigate FGF2's role in the pathogenesis of pulmonary fibrosis.

Main Methods:

  • mRNA sequencing analysis to globally profile FGF2-regulated genes in PFs.
  • Gene Ontology (GO) analysis to identify enriched cellular functions.
  • Gene Set Enrichment Analysis (GSEA) and cellular experiments to validate findings.

Main Results:

  • FGF2 significantly impacts genes involved in extracellular matrix (ECM) organization, cytoskeleton formation, and Wnt signaling.
  • FGF2 suppresses ECM and actin filament organization in PFs.
  • FGF2 promotes PFs proliferation, indicating complex regulatory effects.

Conclusions:

  • FGF2 acts as a key upstream regulator inhibiting pulmonary fibroblast activation.
  • FGF2's modulation of ECM and proliferation suggests a significant role in pulmonary fibrosis.
  • Targeting FGF2 may offer a novel therapeutic strategy for pulmonary fibrosis.
Keywords:
FGF2extracellular matrixmRNA sequencingpulmonary fibroblastsα-SMA

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