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[Characterization of the cellular immune response induced by Mycobacterium tuberculosis Rv2626c]
Guo Li1, Hong Fu1, Yunfei Gao1
1Jiangsu Key Laboratory of Zoonoses, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-fod Safety and Quality, Ministry of Agriculture and Rural Affairs, Joint International Research Laboratory of Agriculture and Agri-product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu, China.
Abstract:
Nearly a quarter of the world's population is infected with Mycobacterium tuberculosis and remains long-term asymptomatic infection. Rv2626c is a latent infection-related protein regulated by DosR of M. tuberculosis. In this study, the Rv2626c protein was prokaryotically expressed and purified, and its immunobiological characteristics were analyzed using RAW264.7 cells and mice as infection models. SDS-PAGE and Western blotting analysis showed that the Rv2626c-His fusion protein was mainly expressed in soluble form and specifically reacted with the rabbit anti-H37RV polyclonal serum. In addition, we found that the Rv2626c protein bound to the surface of RAW264.7 macrophages and up-regulated the production of NO. Moreover, the Rv2626c protein significantly induced the production of pro-inflammatory cytokines IFN-γ, TNF-α, IL-6 and MCP-1, and induced strong Th1-tendency immune response. These results may help to reveal the pathogenic mechanism of M. tuberculosis and facilitate the development of new tuberculosis vaccine.
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