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Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

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Published on: August 3, 2021

Surfactant-Mediated Structural Modulations to Planar, Amphiphilic Multilamellar Stacks.

Daniel J Speer¹, Marta Salvador-Castell², Yuqi Huang³

¹Chemistry Graduate Group, University of California, Davis, One Shields Avenue, Davis, California 95616, United States.

The Journal of Physical Chemistry. B

|August 16, 2023

Summary

This summary is machine-generated.

This study shows how mixing lipids and surfactants creates unique self-assembled structures, revealing new possibilities for biochemical technologies. The research explores how these mixtures form stable lamellar and micellar phases, important for isolating and purifying membrane proteins.

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Published on: January 25, 2018

Area of Science:

Biochemistry and Biophysics
Materials Science
Self-Assembly

Background:

The hydrophobic effect drives amphiphilic self-assembly into lamellar and micellar structures, crucial for biological processes.
Understanding lipid-surfactant interactions is vital for biochemical technologies like membrane protein isolation and purification.

Purpose of the Study:

To investigate the structural organization of mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and two zwitterionic surfactants (DDAPS and O-Lyso-PC).
To explore how water vapor hydration influences the formation and properties of these mixed mesophases.

Main Methods:

X-ray diffraction measurements to analyze structural organization.
Microscopy techniques including brightfield optical, wide-field fluorescence, and atomic force microscopy (AFM) for morphological and topographical analysis.
Assembly of lipid-surfactant mixtures via water vapor hydration.

Main Results:

Multilamellar mesophases formed across a broad range of POPC:surfactant ratios, exceeding classical limits without significant disruption.
Increased surfactant concentration generally decreased lamellar spacing (D) and headgroup-to-headgroup distance (Dhh), with variable water layer thickness (Dw).
AFM revealed homogeneous multilamellar stacks with consistent bilayer thickness and rupture force, irrespective of surfactant concentration.

Conclusions:

Specific chemical interactions between surfactants and POPC, including headgroup hydration and tail mismatch, dictate the structural properties of mixed mesophases.
These findings demonstrate the formation of unique mixed mesophases with tunable structural trends, impacting dissolution pathways of lipid mesophases.
The study highlights how surfactant-lipid interactions can modulate mesophase morphology, offering insights into biochemical separation and reconstitution processes.